Phospho-cdc25C (Thr48) Antibody

• 产品编号：CST-9527S      品牌：CST       原厂货号：9527S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-cdc25C (Thr48) Antibody 能够检测内源性苏氨酸（48位）磷酸化的cdc25C蛋白。该多克隆抗体是由合成的人源的针对苏氨酸（48位）磷酸化肽段免疫动物，采用A蛋白和多肽亲和层析技术生产的。. cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。Cdc25C的完全激活需要12个以上不同位点的磷酸化，这些位点磷酸化依赖于cdc2/cyclin B 和 Polo-样 激酶, 以及肽酰-脯氨酰-异构酶(PPI) Pin1的作用（6,7)。Pin1包含一个结合phospho-Ser/Thr-Pro 位点的WW结构域和一个在phospho-Ser/Thr-Pro结合点诱导顺反式异构化的催化区域(8)。Cdc25C的苏氨酸48和67位点被磷酸化后可以直接与Pin1的WW结构域相互作用(9)。苏氨酸48位的磷酸化也调节cdc25C与CKS/p13SUC1的结合(10)。

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
6. Izumi, T. and Maller, J.L. (1993) Mol. Biol. Cell 4, 1337-1350.
7. Stukenberg, P. T. et al. (2001) Mol. Cell 7, 1071-1083.
8. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
9. Lu, P. J. et al. (1999) Science 283, 1325-1328.
10. Landrieu, I. et al. (2001) J. Biol. Chem 276, 1434-1438.

原厂资料：

Specificity / Sensitivity

Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C only when phosphorylated at Thr48.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr48 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10). Cdc25C的完全激活需要12个以上不同位点的磷酸化，这些位点磷酸化依赖于cdc2/cyclin B 和 Polo-样 激酶, 以及肽酰-脯氨酰-异构酶(PPI) Pin1的作用（6,7)。

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
6. Izumi, T. and Maller, J.L. (1993) Mol. Biol. Cell 4, 1337-1350.
7. Stukenberg, P. T. et al. (2001) Mol. Cell 7, 1071-1083.
8. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
9. Lu, P. J. et al. (1999) Science 283, 1325-1328.
10. Landrieu, I. et al. (2001) J. Biol. Chem 276, 1434-1438.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody