Phospho-Axl (Tyr702) (D12B2) Rabbit mAb

• 产品编号：CST-5724S      品牌：CST       原厂货号：5724S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化的Axl (Tyr702)的兔源单克隆抗体仅在Tyr702被磷酸化后才能检测到Axl的存在。本抗体也能与其它过表达的相关磷酸化酪氨酸激酶（如EGFR）交叉反应。单克隆抗体通过免疫动物得到，该合成磷酸化多肽是根据人的Axl蛋白Tyr702附近的氨基酸序列合成的。Axl、 Sky和 Mer是 受体酪氨酸激酶 (RTK) 家族的三个成员，它们有共同的胞内酪氨酸激酶区域和与胞外粘附分子相似的胞外区。这些受体酪氨酸激酶与维生素K依赖性的生长停滞特异性6蛋白（Gas6）结合，Gas6蛋白与S抗凝血因子蛋白结构上相关(1)。与受体结合后，Gas6能够激活PI3K激酶和它的下游靶分子Akt、 S6K和 NF-κB (2,3)。大量证据表明Gas6/Axl信号通路与正常细胞和癌细胞的生长和存活相关(4)。Axl的Tyr1702位点磷酸化是Cell Signaling Technology (CST)公司利用LC-MS/MS平台PhosphoScan®技术在寻找磷酸化位点过程中发现的(5)。Axl的Tyr702位点磷酸化在某些肿瘤细胞系中存在(6)。

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
5. Rikova, K. et al. (2007) Cell 131, 1190-203.
6. Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein. 

Background

Axl, Sky and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6).

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
5. Rikova, K. et al. (2007) Cell 131, 1190-203.
6. Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody