Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb

• 产品编号：CST-3024L      品牌：CST       原厂货号：3024L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7)兔单克隆抗体可分别识别内源性的Tyr1135/1136磷酸化的IGF-I受体和Tyr1150/1151磷酸化的胰岛素受体。它与其它相关酪氨酸磷酸化的酪氨酸激酶无交叉反应。该单克隆抗体用与人类IGF-I受体β蛋白中Tyr1135/1136位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物制成。I型胰岛素样生长因子受体（IGF-I）是一种跨膜受体酪氨酸激酶，广泛表达于许多细胞系，并在胎儿和产后组织细胞中表达（1-3）。受体与IGF-I和IGF-Ⅱ配体结合后发生自身磷酸化。 激酶结构域的三个酪氨酸残基（Tyr1131，Tyr1135，Tyr1136）是最早确定的三个主要磷酸化位点（4）。三个酪氨酸残基的磷酸化对激酶的活化是必要的（5,6）。胰岛素受体（IR）与IGF-I受体有显著的结构和功能的相似性，都有激酶结构域激活环中对应的酪氨酸残基（Tyr1146/1150/1151）的存在。IR的酪氨酸磷酸化是胰岛素刺激最早的细胞反应之一（7）。自身磷酸化从Tyr1146和Tyr1150或Tyr1151的磷酸化开始，但完全的激酶活性需要三个酪氨酸残基的磷酸化（8）。

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

原厂资料：

Specificity / Sensitivity

Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, respectively. It does not cross-react with other related tyrosine-phosphorylated tyrosine kinases.

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.