Phospho-eIF4E (Ser209) Antibody

• 产品编号：CST-9741P      品牌：Cell Signaling Technology       原厂货号：9741P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-eIF4E (Ser209) Antibody检测仅在serine 209位点磷酸化的内源性eIF4E蛋白水平。通过人工合成人源eIF4E蛋白Ser209位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Eukaryotic initiation factor 4E (eIF4E)结合mRNA帽子结构从而介导翻译的起始(1,2)。eIF4E和eIF4G相互作用，而eIF4G是一个支架蛋白其能促进eIF4E和eIF4A装配到eIF4F复合物上(2)。eIF4B被认为在翻译起始阶段有助于eIF4F复合物的形成。在有丝分裂和Erk、p38 MAPK介导的压力刺激下，体内Mnk1蛋白使eIF4E蛋白在Ser209位点磷酸化(3,4)。在小鼠Mnk1上的两个Erk和p38 MAPK磷酸化位点(Thr197 and Thr202)对Mnk1激酶活性至关重要(3)。eIF4G蛋白羧基末端含有血清刺激的磷酸化位点，包括Ser1108、Ser1148和Ser1192 (5)。通过PI3激酶抑制剂LY294002和FRAP/mTOR通路抑制剂rapamycin可阻断这些位点的磷酸化。

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

原厂资料：

Specificity / Sensitivity

Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.