Phospho-IκBε (Ser18/22) Antibody

• 产品编号：CST-4924S      品牌：CST       原厂货号：4924S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IκBε (Ser18/22) Antibody只能检测内源的在ser18 和ser22 位点磷酸化的IκBε蛋白。此抗体在生理上不与家族的其它成员交叉反应。此多克隆抗体是通过合成人源对应的IκBε ser18/22位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。信号通路的激活通常是通过磷酸化Ser32 和Ser36位点诱导和蛋白水解酶体介导降解IκB的方法激活NF-κB并入核 (3-7)。IκBα的磷酸化和Rel依赖的转录可以经过一系列的胞外的信号激活如炎症因子，生长因子和化学增活素。在这些活性位点磷酸化IκB的激酶已经得到了鉴定(8)。IκBβ 和 IκBε 的调控与 IκBα相似, 然而, 磷酸化和降解这些蛋白从动力学上来讲更慢(9)IκBβ的磷酸化位点是 Ser/Thr19 和 Ser23, 而 IκBε 的磷酸化位点是 Ser18 和 Ser22(10)。

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
9. Hoffmann, A. et al. (2002) Science 298, 1241-1245.
10. Shirane, M. et al. (1999) J. Biol. Chem. 274, 28169-28174.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog

Specificity / Sensitivity

Phospho-IκBε (Ser18/22) Antibody detects endogenous levels of IκBε only when phosphorylated at serines 18 and 22. No cross-reactivity was detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 18/22 of human IκBε. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
9. Hoffmann, A. et al. (2002) Science 298, 1241-1245.
10. Shirane, M. et al. (1999) J. Biol. Chem. 274, 28169-28174.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody