Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb

• 产品编号：CST-2511P      品牌：Cell Signaling Technology       原厂货号：2511P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

只有当丝氨酸（118位）磷酸化时，Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb能够检测内源性水平的雌激素受体α。它不与磷酸化的雌激素受体β发生交叉反应。该单克隆抗体通过用合成磷酸肽免疫动物制备，该合成磷酸肽是人雌激素受体α丝氨酸（118位）附近的残基。雌激素受体α(ERα)是类固醇受体超家族的一员，含有高度保守的DNA结合域（DBD）和配体结合域（LBD）（1）。ERα通过其雌激素非依赖性和雌激素依赖性激活域（分别为AF-1和AF-2），调控转录招募共激活因子蛋白，并与一般的转录机制相互作用（2）。磷酸化提供了调控ERα活性的重要机制（3,4）。 ERα有多个磷酸化位点（5）。丝氨酸（104，106，118和167位）都位于氨基末端的转录激活功能域AF-1，这些丝氨酸的磷酸化在调节ERα活性中起着重要作用。丝氨酸（118位）可能是转录调节激酶CDK7的底物（5）。丝氨酸（167位）可以被p90RSK和Akt磷酸化（4,6）。丝氨酸（167位）的磷酸化可能会使乳腺癌患者对他莫昔芬耐药（4）。

1. Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.
2. Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.
3. Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.
4. Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.
5. Chen, D. et al. (2000) Mol Cell 6, 127-37.
6. Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.

原厂资料：

Specificity / Sensitivity

Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb detects endogenous levels of estrogen receptor alpha only when phosphorylated at serine 118. It does not cross-react with phosphorylated estrogen receptor beta.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser118 of human ER alpha.

Background

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERα activity (3,4). ERα is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).

1. Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.
2. Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.
3. Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.
4. Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.
5. Chen, D. et al. (2000) Mol Cell 6, 127-37.
6. Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.