FUNCTION:SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist. SIZE:1210 amino acids; 134277 Da SUBUNIT:Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. SUBCELLULAR LOCATION:Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY:Expressed in placenta. Isoform 2 is also expressed in ovarian cancers. PTM:Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980]. SIMILARITY:SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. MISCELLANEOUS:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, Ca, H, M
WB, ELISA, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-282
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-phospho-EGFR (Thr654) Antibody, clone 3F2
Alternate Names
ERBB1
ERBB
Product Information
Format
Purified
Control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Presentation
100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Applications
Application
Detect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
Key Applications
Western Blotting
ELISA
Immunoprecipitation
Biological Information
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
Clone
3F2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION:SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist. SIZE:1210 amino acids; 134277 Da SUBUNIT:Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. SUBCELLULAR LOCATION:Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY:Expressed in placenta. Isoform 2 is also expressed in ovarian cancers. PTM:Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980]. SIMILARITY:SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. MISCELLANEOUS:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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