HaloTag? Cloning Starter System

产品编号：G6050 品牌：promega 原厂货号：G6050
产品分类：克隆 > 克隆载体和克隆试剂盒 > 依赖DNA连接酶的克隆试剂盒
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1 each

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2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

Wizard® SV Gel and PCR Clean-Up System【子货号：A9280，包装：1 x 10 preps，，运保温度：室温】

Flexi® System, Entry/Transfer【子货号：C8641，包装：1 x 1 each，，运保温度：-20℃】

HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack【子货号：G3780，包装：1 x 9 × 2μg，，运保温度：-20℃】

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)【子货号：R1901，包装：1 x 50μl，，运保温度：-20℃】

描述：

说明

Flexi^® Vector System(Flexi^® 载体系统) 是一种简单而强大的蛋白编码序列的定向克隆方法。该方法建立在两种位点稀有的限制性酶SgfI 和PmeI 基础上，可以将蛋白编码区在不同的Flexi^® 载体之间转换，而不需要重新测序，该方法快速、有效、保真度高。

所有的Flexi^® 载体带有致死性barnase 基因，该基因可被插入的目的DNA 片段所替代，是连接反应成功的阳性筛选标志。

与其它的位点特异性重组载体系统不同，Flexi^® 载体系统不需要在目的蛋白的氨基或羧基端加上多个氨基酸。此外，该系统不需要archival entry vector( 入门载体)，对于大多数实验而言，都可以找到符合实验设计的载体。

C- 末端Flexi^® 载体可用于带有C- 末端标记物蛋白的表达。这种载体可做为两端带有SgfI 和PmeI 位点的蛋白编码区的接受者，它们缺少PmeI 酶切位点，而带有一个EcoICRI 平端位点。这种组合序列不能从C- 末端Flexi^® 载体中去除，也不能被转移到其它Flexi^®载体上。

特点

. 通用：您可以随意进行众多实验应用( 例如：细菌或哺乳动物蛋白的细胞内表达，无细胞表达)，然后还可以根据需要转向其它实验。

• 省时：高效的转移能够直接利用重组克隆，减少浪费在筛选本底克隆上的时间。

• 提高生产力：对于大规模筛选的需求，可调整至高通量模式。

• 易于获得：不需要许可证书费用，没有复杂的转移限制。

原厂资料：

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-terminal Flexi® Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi® Vectors and transferred to other Flexi® Vectors.

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology Products page.

Features - Benefits

Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian or cell-free protein expression) and then transfer to others as required.
Time Savings: Efficient transfer allows direct use of recombinant clones, minimizing time wasted screening background colonies.
Enhanced Productivity: Adaptable to high-throughput formats for large screening projects.
Easy Access: No licensing fees or complicated transfer restrictions.

Applications

Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

For more information, see the Protocols & Applications Guide.

Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e