The SNAP-Capture Pull Down Resin is used to selectively capture and immobilize a SNAP-tag® fusion protein from solution using agarose beads. These beads have a high loading capacity for SNAP-tag fusion proteins and show very low non-specific absorption of proteins from a complex lysate, making them especially suitable for pull down applications. The SNAP-Capture Pull Down Resin is prepared by the coupling of SNAP-tag substrate benzylguanine with a highly cross-linked agarose (4%) with excellent physical characteristics. Two ml of SNAP-Capture Pull Down Resin is sufficient to perform 25 pull down assays using 80 µl of resin suspension per assay.
The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule or modified surface to a protein of interest. The SNAP-tag is a protein based on mammalian O6-alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the immobilization reaction, the SNAP-tag is covalently attached to the substituted benzyl group of the SNAP-Capture Pull Down Resin.
There are two steps to the use of this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and capture and immobilization of the fusion protein using SNAP-Capture Pull Down Resin.
Materials Required but not Supplied
• Protein sample containing the protein to immobilize expressed as a SNAP-tag fusion
• Buffer for immobilization and washing
• Microcentrifuge
Storage Temperature
4°C
Binding Capacity
SNAP-Capture Pull Down Resin (80 µl) was washed, incubated with 200 µl of 1 mg/ml SNAP-tag CBD (Chitin Binding Domain) for 1 hour at room temperature, then rewashed as described in these instructions. Binding capacity was determined to be 3 mg/ml bed resin.
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