An affinity matrix for the isolation of target proteins fused to a chitin binding domain (CBD) (1). CBD-fusion proteins isolated from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.
Storage Temperature
4°C
Storage Conditions
20% Ethanol in water
Binding Capacity
3 mg chitin binding domain protein / ml bed volume released
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