Geldanamycin

• 产品编号：CST-9843S      品牌：CST       原厂货号：9843S
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 安莎类抗生素
• 应用分类：

描述：

Geldanamycin以600ug粉剂提供。储存在-20℃或以下。使用前将粉末溶解于1.08ml DMSO中成为1mM Geldanamycin。对于工作浓度为300nM-1uM，将原液按1:3000到1:1000稀释。使用需要的浓度处理细胞20-48小时。液体形式储存在-20℃或以下温度。注意：Geldanamycin在液态下不稳定（质量在1-2周后下降）。每次使用geldanamycin时应新鲜配制。Geldanamycin (GA)是一个天然存在的HSP90抑制剂，属于苯醌安沙霉素家族。GA结合HSP90氨基末端的ATP结合口袋，抑制其与ATP的结合和水解。HSP90是一个能与很多肿瘤发生过程中细胞信号和调节相关的重要目标蛋白相互作用的分子伴侣（1,2）。GA结合到HSP90后干扰HSP介导的目标蛋白折叠，导致目标蛋白堆积和降解（1-3）。GA和其合成衍生物对肿瘤细胞HSP90比正常组织有更高的亲和性，使得其成为一种潜在的抗肿瘤药物（2-3）。

1. Ochel, H.J. et al. (2001) Cell Stress Chaperones 6, 105-112.
2. Sharp, S. and Workman, P. (2006) Adv.Cancer Res. 95, 323-348.
3. Kamal, A. et al. (2003) Nature 425, 407-410.

原厂资料：

Directions for Use

Geldanamycin is supplied as 600 μg powder. Store at or below -20ºC. Before usage, dissolve the chemical in 1.08 ml DMSO to make 1 mM Geldanamycin. For working concentrations of 300 nM-1 μM, dilute 1:3000 to 1:1000. Treat cells with the desired concentration for 20-48 hours. Store liquid form at or below -20ºC. 
 Note: Geldanamycin is not stable in liquid form (quality decreased in 1-2 weeks). Use freshly dissolved geldanamycin each time.

Background

Geldanamycin (GA) is a naturally existing HSP90 inhibitor that belongs to the benzoquinone ansamycin family. GA binds to the amino terminal ATP-binding pocket of HSP90 and inhibits ATP binding and hydrolysis. HSP90 is a chaperone interacting with a wide variety of important target proteins for cell signaling and regulation during tumorgenesis (1,2). The binding of GA to HSP90 interferes with HSP-mediated target protein folding, leading to target aggregation and degradation (1-3). GA and its synthetic derivatives show higher affinity to HSP90 in tumor cells as compared to normal tissues and constitute a class of protential antitumor drugs (2-3).

1. Ochel, H.J. et al. (2001) Cell Stress Chaperones 6, 105-112.
2. Sharp, S. and Workman, P. (2006) Adv.Cancer Res. 95, 323-348.
3. Kamal, A. et al. (2003) Nature 425, 407-410.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 1 week to prevent loss of
potency. Aliquot to avoid multiple freeze/thaw cycles.