Ready-to-use solution is ideal for detection of alkaline phosphatase in various procedures, including in situ hybridization and immunoblotting. Also used in immunohistochemistry, producing a reddish-brown color that contrasts well with blue hematoxylin stained sections. Contains 1.15 x 10-3 moles/liter of BCIP and 6.92 x 10-4 moles/liter of INT.