Human Platelet-Derived Growth Factor AA (hPDGF-AA)

• 产品编号：CST-8913SF      品牌：CST       原厂货号：8913SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > PDGF血小板衍生因子
• 应用分类：

描述：

CST公司的人源重组蛋白PDGF-AA (hPDGF-AA) Ser87-Thr211 (Accession #NP_002598)经过大肠杆菌表达纯化获得。重组的hPDGF-AA蛋白在末端没有Met标签，其分子量据推算为14,305。DTT-降解蛋白作为一个18kDa的蛋白而转移，未降解的蛋白由胱氨酸形成二聚体作为一个34kDa的蛋白而转移。重组hPDGF-AA蛋白的N-末端SIEEA的序列通过测序得到。重组蛋白hPDGF-AA的生物活性是通过NIH/3T3细胞的增殖实验确定的。每个lot的ED50值在4-12 ng/ml之间。有载体: 蛋白溶解在20 mM 柠檬酸盐, pH 3.0 包含 100 mM NaCl 和 20 μg BSA/1 μg hPDGF-AA的溶液中，并通过 0.22 μm 滤膜冻干。无载体：蛋白溶解在20 mM 柠檬酸盐, pH 3.0 包含 100 mM NaCl，并通过 0.22 μm 滤膜冻干。PDGF-AA参与到胚胎的发育，血管生成和器官发育和诱导纤维原细胞的增殖和迁移等过程(1,2)。PDGF-AA是通过上皮，肌肉，骨肉瘤神经元细胞而产生(1,3)。活性的PDGF-AA是通过胞内蛋白酶剪切一个很大的前体而形成。PDGF-AA 通过可变剪切产生一个伸长的C-末端集合细胞基质中的成分并得到富集。C-末端的伸长在细胞外被剪切而生成成熟的 PDGF-AA (1,2)。PDGF-AA 与PDGFR-α结合结合激活酪氨酸激酶受体(1)。PDGF-AA诱导的信号传导经过Ras-MAPK, PI3K/AKT 和 PLCγ 通路 (1)。PDGF-AA表达或信号通路的失调通常与癌症和纤维化失调有关系(1)。

原厂资料：

Source / Purification

Recombinant human PDGF-AA (hPDGF-AA) Ser87-Thr211 (Accession #NP_002598) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hPDGF-AA does not have a Met on the amino terminus and has a calculated MW of 14,305. DTT-reduced protein migrates as an 18 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34 kDa protein. The expected amino-terminal SIEEA of recombinant hPDGF-AA was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-AA. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hPDGF-AA was determined in a NIH/3T3 proliferation assay. The ED50 of each lot is between 4-12 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hPDGF-AA.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-AA. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

PDGF-AA is integrally involved in embryonic development, angiogenesis and organogenesis and induces fibroblast proliferation and migration (1,2). PDGF-AA is produced by epithelial, muscle, osteosarcoma and neuronal progenitor cells (1,3). Active PDGF-AA is formed through intracellular proteolytic cleavage of a large precursor. PDGF-AA is also concentrated in the extracellular matrix through alternative splicing that generates an extended carboxy-terminal that binds components of the extracellular matrix. The carboxy-terminal stretch is removed extracellularly to generate mature PDGF-AA (1,2). PDGF-AA binding to the PDGFR-α activates the receptor tyrosine kinase (1). PDGF-AA-induced signaling is through the Ras-MAPK, PI3K/AKT and PLCγ pathways (1). Dysregulation of PDGF-AA expression and/or signaling is often associated with cancer and fibrotic disorders (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.