LongAmp Taq PCR Kit

产品编号：E5200S 品牌：New England Biolabs 原厂货号：E5200S
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 长片段PCR
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
The LongAmp® Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent_R^® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent_R Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. Mg-free buffer is also supplied for situations when complete control over the final magnesium concentration is required to optimize amplification. The kit can generate a wide range of PCR products, up to 30 kb from lambda DNA or from human genomic DNA.

LongAmp Taq DNA Polymerase supplied in: 100 mM KCl, 10 mM Tris-HCl (pH 7.4@ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween 20, 0.5% NP-40 and 50% Glycerol.

1X LongAmp® Taq DNA Polymerase Reaction Buffer:
60 mM Tris-SO₄ (pH 9.0 @ 25°C)
20 mM (NH₄)₂SO₄
2 mM MgSO₄
3% glycerol
0.06% NP-40
0.05% Tween-20

Applications:

Long Range PCR

Advantages:

Offers two fold higher fidelity than Taq DNA Polymerase alone
Allows for robust amplification of PCR products up to 30 kb from low complexity templates and up to 30 kb from difficult templates, like human genomic DNA

Kit Components:
dNTP Mix (10 mM)
LongAmp® Taq (Mg-free) Reaction Buffer Pack (5X)
LongAmp® Taq DNA Polymerase (2,500 units/ml)
LongAmp® Taq Reaction Buffer Pack (5X)
MgSO₄ (100 mM)
Nuclease Free dH₂O
Crimson LongAmp® Taq Reaction Buffer Pack