Description:
LongAmp® Taq DNA Polymerase is a unique blend of Taq and Deep VentR™ DNA Polymerases. The 3´→ 5´ exonuclease activity of Deep VentR DNA Polymerase increases the fidelity and robust amplification of Taq Polymerase (1).
Crimson LongAmp® Taq DNA Polymerase combines the robust LongAmp® Taq DNA Polymerase with a colored reaction buffer. Crimson LongAmp Taq DNA Polymerase can amplify up to 20 kb with minimal or no optimization from DNA samples of both low complexity (i.e. plasmid) and high complexity (i.e. genomic DNA). Maximum amplicon sizes are 30 kb from lambda DNA or from human genomic DNA. It offers three unique features, including a color indicator for reaction setup, direct loading of PCR product onto a gel and a tracking dye during electrophoresis.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
Heat Inactivation:
No Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions: 1X Crimson LongAmp Taq Reaction Buffer, DNA template, primers, 300 µM dNTPs (not included) and 5 units of Crimson LongAmp Taq DNA Polymerase in a total reaction volume of 50 µl.
1X Crimson LongAmp Taq Reaction Buffer:
60 mM Tris-SO4
20 mM (NH4)2SO4
2 mM MgSO4
3% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween-20
Acid Red
pH 9.0 @ 25°C
Concentration:
2,500 units/ml
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% NP-40
pH 7.4 @ 25°C
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