Raised against histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH conjugated synthetic peptide.
Conjugate
Unconjugated
Form
Frozen
Concentration
Lot-Specific
Storage buffer
whole serum
Preservative
<0.1% sodium azide
Storage Conditions
-20° C, Avoid Freeze/Thaw Cycles
Tested Applications
Dilution *
ChIP assay (ChIP)
1 ug
ELISA (ELISA)
1:100 to 1:500
Immunocytochemistry (ICC)
1:200
Immunofluorescence (IF)
1:200
Western Blot (WB)
1:250
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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