描述：
MDM2, originally described as a gene product of mouse double minute chromosomes, is a nuclear oncoprotein that can inhibit the action of
certain tumor suppressor proteins. For example, MDM2 binds to the acidic activation domain (residues 2042) of the p53 tumor suppressor
protein, and the p53-MDM2 complex down regulates the transcriptional activity of p53. p53 plays a role in the normal cell cycle by activating
the transcription of genes that cause arrest in G1. The expression of MDM2 is itself, induced by p53 and may be a way for p53 to self-regulate
its activity during the normal cell cycle. However, overexpression of MDM2 results in the loss of p53-regulated growth control and
consequently, deregulated cell proliferation. MDM2 also binds to the Retinoblastoma tumor suppressor protein (Rb) and inhibits its growth
regulatory function. MDM2 can directly augment proliferation by binding to two transcription factors E2F1 and DP1, and stimulating the
activity of the S-phase inducing E2F1/DP1 heterodimer. MDM2 migrates at a reduced molecular weight of ~95 kDa.
The SMP14 clone has been reported to recognize human, mouse and rat MDM2 while exhibiting a slight cross-reactivity with cytokeratins 6,
14 and 16 in some experimental systems. In the immunoprecipitation application, SMP14 has been reported to precipitate MDM2 and
p53-MDM2 complexes. MCF7 human breast carcinoma cells (ATCC HTB-22) and NIH/3T3 mouse fibroblasts (ATCC CRL-1658) are
suggested as western blot and immunoprecipitation positive controls. SMP14 has been reported to be useful for the immunohistochemical
staining of acetone-fixed, frozen sections and of formalin-fixed, paraffin-embedded tissue sections. In addition to a nuclear staining of
MDM2, cytoplasmic staining may also be observed which is likely to be atrributable to the slight crossreactivity of the SMP14 clone with
cytokeratins.
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.
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