T3 RNA POLYMERASEEA

产品编号：EP0102 品牌：invitrogen 原厂货号：EP0102
产品分类：克隆 > 聚合酶 > RNA聚合酶
应用分类：

包装：

EA

运保温度:

-20°C

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其它组分：

保存缓冲液组分：50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM燚TT, 0.1 mg/ml BSA, 0.03% (v/v) ELUGENT变性剂, 50% (v/v)甘油

5X 转录缓冲液：200 mM Tris-HCl (pH 7.9, 25°C), 30 mM燤gCl2, 50 mM DTT, 50 mM NaCl, 10 mM亚精胺

描述：

Thermo Scientific Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.

Highlights

Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Applications

Synthesis of unlabeled and labeled RNA that can be used:
- For hybridization,in vitro RNA translation
- As aRNA , siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
- In studies of RNA secondary structure and RNA-protein interactions RNA splicing

原厂资料：

Thermo Scientific Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.

Highlights

Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Applications

Synthesis of unlabeled and labeled RNA that can be used:
- For hybridization,in vitro RNA translation
- As aRNA , siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
- In studies of RNA secondary structure and RNA-protein interactions RNA splicing

注意事项：

Consensus promoter sequences:

T3	`AATTAACCCTCACTAAAGGGAGA`
T7	`TAATACGACTCACTATAGGGAGA`
SP6	`ATTTAGGTGACACTATAGAAGNG`

The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively .