SP6 RNA Polymerase

• 产品编号：M0207S      品牌：New England Biolabs       原厂货号：M0207S
• 产品分类：克隆 > 聚合酶 > RNA聚合酶
• 应用分类：

描述：

• Isolated from a recombinant source

• RNA probe preparation for hybridization

• mRNA generation for in vitro translation systems

• Supplied with 10X Reaction Buffer

Description:
SP6 RNA Polymerase catalyzes the synthesis of RNA in the 5´→ 3´ direction in the presence of a DNA template containing an SP6 phage promoter. SP6 RNA Polymerase can be used to generate high specific activity labeled RNA probes, RNA for in vitro translation, biologically active mRNA and/or preparative quantities of defined length RNA by run off transcription (1).

Source:
An E. coli strain that carries the cloned gene for SP6 RNA Polymerase from Salmonella typhimurium LT2Z.

Applications:

• Radiolabeled RNA probe preparation (2)
• RNA generation for in vitro translation (3)
• RNA generation for studies of RNA structure, processing and catalysis (3)
• Expression control via anti-sense RNA

Reagents Supplied:
RNAPol Reaction Buffer (10X)

Reaction Conditions:
1X RNAPol Reaction Buffer
Supplemented with 0.5 mM ATP, 0.5 mM Guanosine triphosphate, 0.5 mM UTP, and 0.5 mM CTP
Incubate at 40°C.

1X RNAPol Reaction Buffer:
40 mM Tris-HCl
6 mM MgCl₂
10 mM Dithiothreitol
2 mM spermidine
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 40°C in 1X RNA Polymerase Reaction Buffer.

Concentration:
20,000 units/ml

Storage Conditions:
50 mM Tris-HCl
100 mM NaCl
20 mM 2-Mercaptoethanol
1 mM EDTA
50% Glycerol
0.1% Triton X-100
pH 7.9 @ 25°C

 

原厂资料：

• Isolated from a recombinant source

• RNA probe preparation for hybridization

• mRNA generation for in vitro translation systems

• Supplied with 10X Reaction Buffer

Description:
SP6 RNA Polymerase catalyzes the synthesis of RNA in the 5´→ 3´ direction in the presence of a DNA template containing an SP6 phage promoter. SP6 RNA Polymerase can be used to generate high specific activity labeled RNA probes, RNA for in vitro translation, biologically active mRNA and/or preparative quantities of defined length RNA by run off transcription (1).

Source:
An E. coli strain that carries the cloned gene for SP6 RNA Polymerase from Salmonella typhimurium LT2Z.

Applications:

• Radiolabeled RNA probe preparation (2)
• RNA generation for in vitro translation (3)
• RNA generation for studies of RNA structure, processing and catalysis (3)
• Expression control via anti-sense RNA

Reagents Supplied:
RNAPol Reaction Buffer (10X)

Reaction Conditions:
1X RNAPol Reaction Buffer
Supplemented with 0.5 mM ATP, 0.5 mM Guanosine triphosphate, 0.5 mM UTP, and 0.5 mM CTP
Incubate at 40°C.

1X RNAPol Reaction Buffer:
40 mM Tris-HCl
6 mM MgCl₂
10 mM Dithiothreitol
2 mM spermidine
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 40°C in 1X RNA Polymerase Reaction Buffer.

Concentration:
20,000 units/ml

Storage Conditions:
50 mM Tris-HCl
100 mM NaCl
20 mM 2-Mercaptoethanol
1 mM EDTA
50% Glycerol
0.1% Triton X-100
pH 7.9 @ 25°C