ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR (1). It is engineered to have high thermal stability (up to 70°C) (Figure 1), copy RNA with secondary structure, and improve priming specificity (2). ThermoScript™ Reverse Transcriptase offers:
Greater first-strand cDNA yields than reverse transcriptases with RNase H activity
More full-length cDNA synthesis than reverse transcriptases with RNase H activity
Flexibility–uses either total or poly(A) + RNA
The ability to use RNA primed with oligo(dT), random primers, or a gene-specific primer
Generation of RT-PCR products up to 12 kb
Activity up to 70°C
Application: Synthesis of first-strand cDNA for RT-PCR (1).Unit Definition: One unit of ThermoScript™ is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)25 as template•View Moreprimer.Source: Purified from a baculovirus clone.
原厂资料：
ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR (1). It is engineered to have high thermal stability (up to 70°C) (Figure 1), copy RNA with secondary structure, and improve priming specificity (2).
•Yield– Reduced RNase H activity results in greater first-strand cDNA yields •Size of cDNA– Generation of RT-PCR products up to 12 kb •Thermostability– ThermoScript™ Reverse Transcriptase is active up to 70°C •Flexibility– Uses either total or poly(A)+RNA
Unit Definition: One unit of Platinum®TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
注意事项：
Store at -70°C. Guaranteed stable for 6 months when properly stored.
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