描述：
TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is:
•Gentle on cells - maintains cell health for reproducible results
•Room-temperature stable - convenient to use
•Easy to use - substitutes directly into existing protocols, with no inactivation required
•Animal origin free (AOF) - pure, consistent, and effective
This TrypLE™ Express is modified as follows:
With Without
• EDTA • Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Gentle on cells
TrypLE™ Express, like trypsin, cleaves peptide bonds on the C-terminal side of lysine and arginine. However, TrypLE™ Express‘ exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE™ Express is room-temperature stable and ready to use. TrypLE™ Express cell dissociation reagents remain stable for 24 months at room temperature or 2-8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Express can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Express is free of animal-derived components.
Product Intended Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® TrypLE™ Express is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
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