TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:
• Gentle on cells—maintains cell health for reproducible results • Room-temperature stable—convenient to use • Easy to use—substitutes directly into existing protocols, with no inactivation required • Animal origin free (AOF)—pure, consistent, and effective
This TrypLE™ Select is modified as follows:
With
Without
• EDTA
• Phenol Red
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF) Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
原厂资料：
注意事项：
Storage conditions: 15–30°C. Alternate storage conditions: 2–8°C or -5 to -20°C. Shipping conditions: Ambient. Shelf life: 24 months from date of manufacture.
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