Phire Reaction Buffer

• 产品编号：F-524L      品牌：Finnzymes       原厂货号：F-524L
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The 5X buffer contains 1.5 mM MgCl2 at final reaction concentration. A separate tube of DMSO is provided for further optimization.

Includes

Phire Reaction Buffer (F-524):
• 5X Phire Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phire Reaction Buffer, Detergent-free (F-525):
• Detergent-free 5X Phire Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

原厂资料：