Phusion HF Buffer Pack

• 产品编号：F-518S      品牌：Finnzymes       原厂货号：F-518S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Includes

Phusion HF Buffer Pack (F-518):
• 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phusion GC Buffer Pack (F-519):
• 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phusion Green HF Buffer Pack (F-538L):
• 5X Phusion Green HF Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phusion Green GC Buffer Pack (F-539L):
• 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phusion HF Buffer Pack, Detergent-free (F-520):
• Detergent-free 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Phusion GC Buffer Pack, Detergent-free (F-521):
• Detergent-free 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

原厂资料：