NucBlue® Live 647 ReadyProbes® Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.
• No need to dilute, weigh, or pipette • Convenient dropper bottle—just use two drops per mL • Stable at room temperature—keep handy at your scope or cell culture area • Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm
Cell imaging applications The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red®.
Suggestions for use • NucRed® Live 647 ReadyProbes® Reagent may be added directly to cells in full media, or buffer solutions. • In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity. • In most cases, staining intensity increases with time if cells are not washed prior to imaging. • It is detected through a far-red filter, such as a Cy®5 filter, or a Cy®5 Light Cube for EVOS® imaging systems.
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For Research Use Only. Not for use in diagnostic procedures.
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