7-AAD Staining Solution 2mL

产品编号：BD-559925 品牌：BD-Pharmingen 原厂货号：559925
产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
应用分类：核酸分离纯化与分析 > 核酸凝胶电泳与印迹 > DNA染料

包装：

2mL

运保温度:

Store undiluted at 4°C

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描述：

7-氨基放线菌素D（7-AAD）是一个方便的、现成的核酸染料，它可代替碘化丙啶（PI），用于在FACS分析中使死细胞染色。7-AAD优于PI之处在于，可与藻红蛋白（PE）和异硫氰酸荧光素（FITC）标记的单抗相结合，用于FACS双染分析，因为这三种染料的光谱重叠区域最少。7-AAD荧光可在远红外光谱范围内（650纳米波长）被检测到。该试剂可用于在死细胞群众筛选活细胞。该试剂使用前不用稀释，建议用量：作用于对死细胞排除方法生存能力探针，基于光散射和7-AAD摄取如FL3检测。此试剂不需要稀释。建议使用为：每个反应使用5微升（0.25微克）/（1×10^6个细胞），孵育10分钟后进行FACS分析。

原厂资料：

7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter). This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3. This reagent does not require dilution. Suggested quantity to use: 5 μl (0.25 μg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.

注意事项：

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).
2.Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in plumbing.

4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.