PCDNA 3.4 TOPO CLONING KIT 10 REACTIONS

• 产品编号：A14697      品牌：invitrogen       原厂货号：A14697
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：

描述：

The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO® vector contains the following elements:

• Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
• WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
• TOPO® cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
• Neomycin resistance gene for selection of stable cell lines with Geneticin®
• pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO® TA Cloning Kit includes TOPO®-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot® TOP10 competent cells.
 

原厂资料：

注意事项：

Store pcDNA™3.4-TOPO® vector and all cloning reagents at -20°C.
Store One Shot® TOP10 Chemically Competent E. coli cells at -80°C.