Phosphopeptide corresponding to amino acids 688–698 of human STAT5a
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
3µg
Flow Cytometry (Flow)
2-4µg x 10^6 cells
Immunocytochemistry (ICC)
2-4µg
Immunofluorescence (IF)
2-4µg
Immunohistochemistry (Paraffin) (IHC (P))
1:20-1:200
Western Blot (WB)
2-5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
STAT5, consisting of isoform a and b, belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. STAT5a is activated by IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones. In humans, the STAT5a gene is located on chromosome 17.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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