MitoTracker^? Red CMXRos

• 产品编号：CST-9082S      品牌：CST       原厂货号：9082S
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞结构探针 > 基于荧光染料的细胞结构探针
• 应用分类：

描述：

每小瓶MitoTracker® Red CMXRos包含50 μg冻干的固体。对于1 mM浓度贮存液，在冻干粉中加入94.1 μl 高品质的DMSO。最佳染色浓度和孵育时间根据应用的不同而有所不同。将贮存液直接用生长培养基稀释到浓度为15-125nm，37ºC培养15-45分钟后，可获得典型的结果。培养过后，将细胞用冰冷的甲醇在-20ºC固定15分钟，再用PBS洗3次，每次5分钟。然后开始进行免疫染色。MitoTracker® dyes是一种细胞可透过的探针，包含一个轻度巯基活性的氯甲基部分来进行线粒体的标记。为了标记线粒体，只需将细胞与MitoTracker® probes一起培养，然后探针就会被动扩散，穿过质膜，在有活性的线粒体中积聚。

原厂资料：

Directions for Use

Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into growth media at a concentration between 15-125 nM and incubation between 15-45 min at 37ºC. After incubation, fix the cells in ice-cold methanol for 15 min at -20ºC then rinse 3 times with PBS for 5 min. Proceed with immunostaining.

注意事项：

Storage: Store lyophilized solid at -20ºC dessicated and protected
from light. In lyophilized form, this reagent is stable for 6
months. Once reconstituted in DMSO, the solution can be stored
at -20ºC, protected from light and must be used within 2 weeks.
Avoid freeze-thaw cycles.