Human Fibroblast Growth Factor 9 (hFGF9)

• 产品编号：CST-12296LF      品牌：CST       原厂货号：12296LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > FGF 成纤维细胞生长因子
• 应用分类：

描述：

Cell Signaling Technology使用E.coli表达重组人FGF9 (hFGF9) Leu4-Ser208 蛋白(Accession #NP_31371)。重组hFGF9分子量为23,141 Da.DTT-还原的和未还原的蛋白迁移率为23 kDa多肽。重组hGFG9的氨基端序列由氨基酸测序确认。通过SDS-PAGE确认6 μg还原（+）和未还原（-）重组hFGF9纯度大于>95%。所有的批次纯度都大于>95%。使用NIH/3T3细胞扩增assay检测hFGF9的生物活性。每个批次的ED50都在0.05-0.80 ng/ml之间。带载体：hFGF9 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hFGF9经过0.22 μm过滤后冻干。无载体：hFGF9 in 20 mM Tris, pH 7.2经过0.22 μm过滤后冻干。成纤维细胞生长因子(FGF)9是FGF家族蛋白一员，在发育，癌症和代谢过程中发挥关键作用。FGF9与受体结合需要肝素的作用诱导受体二聚化，随后转磷酸化，下游激活Erk, Akt, 和 PLCγ信号通路(1). FGF9在器官发育和骨骼修复时有重要作用（2-4）。

原厂资料：

Source / Purification

Recombinant human FGF9 (hFGF9) Leu4-Ser208 (Accession #NP_31371) was expressed in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hFGF9 has a calculated MW of 23,141 Da. DTT-reduced and nonreduced protein migrates as a 23 kDa polypeptide. The expected amino terminus of recombinant hFGF9 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hFGF9. All lots are greater than 95% pure.

Bioactivity

The bioactivity of hFGF9 was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05-0.80 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hFGF9.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hFGF9. Carrier free: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2.

Background

Fibroblast growth factor (FGF) 9 is a member of the larger FGF family of proteins that play key roles in development, cancer, and metabolism. Binding of FGF9 to its receptor requires interaction with heparin and induces receptor dimerization, subsequent transphosphorylation, and downstream activation of Erk, Akt, and PLCγ pathways (1). FGF9 is important for organ development and bone repair (2-4).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.