Chymotrypsin, Sequencing Grade

• 产品编号：V1062      品牌：promega       原厂货号：V1062
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白酶 > 胰蛋白酶
• 应用分类：蛋白质研究 > 质谱分析 > 质谱样品制备

其它组分：

描述：

胰凝乳蛋白酶(Chymotrypsin) 是一种高纯度丝氨酸胞内蛋白酶，来源于牛胰腺，可以优先水解芳香族氨基酸( 酪氨酸、苯丙氨酸和色氨酸) 的羧基端。据观察，亮氨酸和甲硫氨酸也可被该酶剪切，但效率较低。胰凝乳蛋白酶在pH 范围7.0-9.0 时活性最佳。该测序级的酶可单独使用，也可以与其它蛋白酶一起使用，经过消化的蛋白可用于肽图谱，也可用于通过肽质谱指纹印迹或MS/MS 光谱匹配进行蛋白鉴定。消化反应既可以在溶液中进行，也可以在凝胶中进行。

原厂资料：

Chymotrypsin is a highly-purified serine endopeptidase derived from bovine pancreas that preferentially hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

Applications

Peptide mapping.
Protein identification.

注意事项：