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PNGase F

 
包装: 500u
运保温度: +2° to +10°C
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PNGase F【子货号:V483A,包装:1 x 500 units,,运保温度:+2° to +10°C】


描述:

 

PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. PNGase F has a molecular weight of 36kDa. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (Figure 1). PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.

Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One Promega unit is equal to 1 IUB milliunit.

Molecular Weight: PNGase F has a molecular weight of approximately 36kDa.

Physical Form: PNGase F is supplied as a liquid in 20mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml.

Applications

  • Characterizing whether the protein is glycosylated (Figure 2).
  • Determining the location of glycosylation on the protein (Figure 3).
  • Characterizing the glycan structure.
  • Protein trafficking.


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