Mung Bean Nuclease

产品编号：M4311 品牌：promega 原厂货号：M4311
产品分类：克隆 > DNA/RNA修饰酶 > 核酸酶
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包装：

运保温度:

–20°C

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其它组分：

Mung Bean Nuclease【子货号：M431A，包装：1 x 2,000u，，运保温度：–20°C】

Mung Bean Nuclease 10X Reaction Buffer【子货号：M432A，包装：1 x 1ml，，运保温度：–20°C】

描述：

说明

Mung Bean Nuclease( 绿豆核酸酶) 以内切方式催化单链DNA 及RNA 的降解并产生以5`- 磷酸为末端的产物。该酶降解单链DNA 的效率比降解双链DNA 的效率高30,000 倍，从两端降解双链DNA 需要很高的酶浓度。Mung Bean Nuclease 被用于转录图谱的研究、补平粘性末端、分离以逆转录酶和DNA 聚合酶Ⅰ合成的cDNA。

特点

• 提供10× 反应缓冲液：300mM NaAC (pH 5.0,15℃ )，500mM NaCl，10mM ZnCl₂。

原厂资料：

Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I.

Features - Benefits

Provided with 10X Reaction Buffer: 300mM sodium acetate (pH 5.0 at 15°C), 500mM NaCl, 10mM ZnCl₂.

Applications

Removal of protruding single-stranded termini in double-stranded DNA.
Selective cleavage of single-stranded nucleic acid, e.g., mRNA mapping experiments.

References

Ardelt, W. and Laskowski, M., Sr. (1971) Biochem. Biophys. Res. Comm. 44, 1205–11.
Kroeker, W.D. et al. (1976) Biochemistry 15, 4463–7.
Mathis, D.J. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 7383–7.
Green, M.R. and Roeder, R.G. (1980) Cell 22, 231–42.
Gubler, U. (1987) Meth. Enzymol. 152, 330–5.