Exonuclease III

产品编号：M1811 品牌：promega 原厂货号：M1811
产品分类：克隆 > DNA/RNA修饰酶 > 核酸酶
应用分类：

包装：

5000u

运保温度:

-20℃

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其它组分：

Exonuclease III 10X Buffer【子货号：E577B，包装：1 x 1ml，，运保温度：-20℃】

Exonuclease III【子货号：M181A，包装：1 x 5,000u，，运保温度：-20℃】

描述：

说明

Exonuclease III( 核酸外切酶III) 是一种特异的双链DNA 3` → 5`核酸外切酶。该酶催化从缺口、平端、凹端及少于4 个碱基的3`- 突出端的3`-OH 处将单核苷酸逐个去除的反应，产生5`- 磷酸核苷。Exonuclease III 具有内在的3`- 磷酸酶活性，因此也可从3`- 磷酸末端降解DNA。另外，该酶还具有类脱嘌呤核酸内切酶活性及核糖核酸酶H 活性。Exonuclease III 与S1 核酸酶联用，可从DNA 片段的末端单向删除序列。

特点

• 灵活：通过改变孵育温度来控制降解速度。

• 高温失活：Exonuclease III 在75℃加热10 分钟失活。

• 提供10× 反应缓冲液：660mM Tris-HCl (pH 8.0，25℃ )，6.6mM MgCl₂。

原厂资料：

Exonuclease III is a 3´→5´ exonuclease specific for double-stranded DNA. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. Exonuclease III will also degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase activity. In addition, the enzyme has apurinic endonuclease activity and ribonuclease H activity. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments.

Features - Benefits

Flexible: Control deletion rate by varying incubation temperature.
May Be Heat-Inactivated: Exonuclease III may be inactivated by heating to 75°C for 10 minutes.
Provided with 10X Reaction Buffer: 660mM Tris-HCl (pH 8.0 at 25°C), 6.6mM MgCl₂.

Applications

Generating nested sets of deletions with double-stranded, linear DNA.
Preparation of single-stranded DNA.

References

Weiss, B. (1976) J. Biol. Chem. 251, 1896–901.
Rogers, S.G. and Weiss, B. (1980) Meth. Enzymol. 65, 201–11.
Henikoff, S. (1984) Gene 28, 351–9.
Promega Product Information #9PIM181, Promega Corporation.