HaloTag? Mammalian Pull-Down System

产品编号：G6504 品牌：promega 原厂货号：G6504
产品分类：蛋白质检测与分析 > 蛋白质相互作用分析 > 免疫共沉淀
应用分类：

包装：

24 reactions

运保温度:

到货周期：

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其它组分：

10X TBS Buffer【子货号：G650A，包装：1 x 25ml，，运保温度：室温】

SDS Elution Buffer【子货号：G651A，包装：1 x 1.3ml，，运保温度：室温】

Mammalian Lysis Buffer【子货号：G938B，包装：1 x 10ml，，运保温度：4 ℃】

HaloLink™ Resin【子货号：G1912，包装：1 x 1.25ml，，运保温度：4 ℃】

Protease Inhibitor Cocktail, 50X【子货号：G652A，包装：1 x 1ml，，运保温度：-20℃】

描述：

说明

HaloTag^® Mammalian Pull-Down and Labeling System(HaloTag^®哺乳动物Pull-Down 和标记系统，Cat.# G6500和G6504) 用于捕获和纯化细胞内成对的和高等级的蛋白复合物，包括瞬时的或弱相互作用的伴侣蛋白。该系统包含 HaloTag^®TMRDirect™ Ligand(HaloTag^®TMRDirect™ 配基)，可以用同一种载体研究相关的细胞定位和实时成像，以进一步理解蛋白的全部功能。而HaloTag^® Mammalian Pull-Down System 不包含HaloTag^®TMRDirect™配基。

依赖于人巨细胞病毒(CMV) 即刻早期增强子、T7 或SP6 RNA聚合酶启动子，HaloTag^®Control Vector (HaloTag^® 对照载体) 可以在哺乳动物细胞、大肠杆菌或体外表达系统中表达HaloTag^®蛋白。它可用作任何HaloTag^® 实验系统的对照，也可用于哺乳动物细胞中HaloTag^® 的稳定和瞬时表达；如想获得HaloTag^® 的稳定表达，共转染的载体需要含有选择标记。

Protease Inhibitor Cocktail, 50X ( 蛋白酶抑制剂混合物，50 X)是针对不同靶标蛋白酶的六种不同蛋白酶抑制剂的混合物。该产品以冻干的形式提供，可用100% 乙醇或DMSO 重悬。

Mammalian Lysis Buffer ( 哺乳动物裂解缓冲液) 是为HaloTag^®哺乳动物表达系统[ 如HaloTag^® Mammalian Pull-Down and LabelingSystems 以及HaloCHIP™ System (Cat.# G9410)] 而设计的，配方中含有50mM Tris-HCl ，150mM NaCl，1% Triton^®X-100 和0.1%脱氧胆酸钠(pH 7.5)。

特点

• 直接从裂解液中快速、高效地共价捕获成对的和高等级的蛋白复合物：增强了对蛋白伴侣的捕获，包括瞬时相互作用。

• 纯度高，背景低：提高了蛋白鉴定的精度；共价连接使得诱饵蛋白在需要的时候可以保留下来。

• 能用荧光标记相同的基因融合物：将复合物捕获和细胞定位联系起来。

• 与下游所有的分析方法都兼容：可自由地在各种应用中鉴定复合物，包括质谱。

原厂资料：

The HaloTag® Mammalian Pull-Down Systems (Cat.# G6500, G6504 and G6509) are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners.

HaloTag® Mammalian Pull-Down System (Cat.# G6504) includes buffers and resin necessary to perform a HaloTag® pull-down.

HaloTag® Mammalian Pull-Down and Labeling System (Cat.# G6500) includes everything in G6504 plus the HaloTag® TMRDirect™ Ligand, which allows correlative cellular localization and real-time imaging studies.

HaloTag® Complete Pull-Down System (Cat.# G6509) includes everything in G6500 plus a starter cloning system, Wizard® SV Gel and PCR Clean-Up System, and FuGENE® HD Transfection Reagent.

The HaloTag® Control Vector provides protein expression of the HaloTag® protein in mammalian cells, E. coli or in vitro expression systems dependent on human cytomegalovirus (CMV) intermediate early enhancer, T7 or SP6 RNA polymerase promoters. It can be used as a control for any HaloTag® experimental system and can be used for both stable and transient HaloTag® expression in mammalian cells; for stable expression, co-transfection with a vector containing a selectable marker is required.

The Protease Inhibitor Cocktail, 50X, is a mixture of six different protease inhibitors with different target protease specificities. This product is provided in a freeze-dried format and can be reconstituted using either 100% ethanol or DMSO.

The Mammalian Lysis Buffer is designed for use with HaloTag® Mammalian-based expression systems such as the HaloTag® Mammalian Pull-Down and Labeling Systems (referenced here) as well as the HaloCHIP™ System (Cat.# G9410). Formulation consists of 50mM Tris-HCl, 150mM NaCl, 1% Triton® X-100 and 0.1% sodium deoxycholate (pH 7.5).

Related Services: Mass Spec Services.

Features - Benefits

Rapid, Efficient and Covalent Capture of Binary and Higher Order Complexes Directly from Lysates: Improved capture of protein partners, including transient interactions.
High Purity and Low Background: Improved accuracy in identification of proteins; covalent attachment allows bait protein to remain behind if desired.
Ability to Fluorescently Label the Same Genetic Fusion: Correlate complex capture with cellular localization.
Compatibility with All Downstream Methods of Analysis: Freedom to identify complexes in variety of applications including mass spectrometry.