The DeadEnd™ Fluorometric TUNEL System is a classic TUNEL Assay designed for the specific detection and quantitation of apoptotic cells within a cell population. The DeadEnd™ Fluorometric TUNEL System measures nuclear DNA fragmentation, an important biochemical hallmark of apoptosis in many cell types. The system is non-radioactive and provides simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level or in cell suspensions. The DeadEnd™ Fluorometric TUNEL System measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3´-OH DNA ends using the enzyme Terminal Deoxynucleotidyl Transferase (TdT), which forms a polymeric tail using the principle of the TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. The fluorescein-12-dUTP-labeled DNA can then be visualized directly by fluorescence microscopy or quantitated by flow cytometry.
Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.# M6101) can be used to generate the positive control and is available separately.
Features - Benefits
Save Money: System provides sufficient reagents for 60 assays of 50μl each.
Save Time: Direct incorporation of fluorescent nucleotide reduces number of incubation steps.
Choose Sample Type: Use to detect apoptosis in cultured cells and formalin-fixed, paraffin-embedded tissue sections.
Convenient: Plastic coverslips provided simplify sample handling.
注意事项：
储存于 -20℃。核苷混合物需避光储存于 -20℃。
Store at –20°C. Store the Nucleotide Mix protected from light at –20°C.
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