CREB Consensus Oligonucleotide

• 产品编号：E3282      品牌：promega       原厂货号：E3282
• 产品分类：DNA和RNA纯化 > DNA纯化 > DNAs
• 应用分类：

其它组分：

描述：

电泳迁移率分析(EMSA, 凝胶迁移实验，凝胶阻滞) 是用于研究蛋白: DNA 相互作用的相对简单而灵敏的方法。这些寡核苷酸含有与特定转录因子结合的DNA 共有序列，而这些转录因子是DNA序列特异的。双链寡核苷酸被设计为具有5` 0H 平末端，易于使用T4 多聚核苷酸激酶进行高特异活性标记。

原厂资料：

The gel shift, or electrophoretic mobility shift, assay provides a simple and rapid method for detecting DNA-binding proteins. Promega has developed the Gel Shift Assay System, which contains target oligonucleotides, a control extract containing DNA-binding proteins, binding buffer and reagents for phosphorylating oligonucleotides. The Gel Shift Assay Core System includes sufficient HeLa nuclear extract to perform 20 control reactions, Gel Shift Binding 5X Buffer, an SP1 Consensus Oligo and an AP2 Consensus Oligo. This is a reliable system for obtaining experience with gel shift assays because AP2 binding activity is stable and produces a strong gel shift.

注意事项：

储存于-20℃。