pCMVTNT? Vector

• 产品编号：L5620      品牌：promega       原厂货号：L5620
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 无细胞表达载体
• 应用分类：蛋白质研究 > 蛋白质表达 > 无细胞表达 > 无细胞表达载体

其它组分：

描述：

原厂资料：

The pCMVTnT™ Vector is designed for the convenient expression of cloned genes using both in vivo and in vitro expression systems. Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple cloning site. This allows for gene expression from either an SP6- or T7-based coupled in vitro transcription/translation system. The presence of RNA phage promoters also allows for the highly efficient synthesis of RNA in vitro. The pCMVTnT™ Vector also contains a 5´ β-globin leader sequence that has been referenced for enhanced expression of certain genes in vitro. For in vivo expression, the vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell types. A β-globin/IgG chimeric intron is located downstream from the enhancer/promoter region. The late SV40 polyadenylation site is located downstream of the multiple cloning site.

Features - Benefits

• In Vivo Expression: The CMV enhancer/promoter region allows strong constitutive expression in many cell types.
• Flexible: The vector contains tandem SP6 and T7 phage promoters allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

References

1. Falcone, D. and Andrews, D.W. (1991) Mol. Cell. Biol. 11, 2656–64.
2. Annweiler, A. et al. (1991) Nucl. Acids Res. 19, 3750.
3. Schmidt, E.V. et al. (1990) Mol. Cell. Biol. 10, 4406–11.
4. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
5. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.