FREESTYLE 293 EXPRESSION SYS EA

• 产品编号：K900001      品牌：invitrogen       原厂货号：K900001
• 产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 哺乳动物表达系统
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描述：

The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.

FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression 查看更多Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.

原厂资料：

注意事项：

The FreeStyle™ 293 Expression System contains:

• 1L GIBCO® FreeStyle™ 293 Expression Medium

• 1 x 10⁷ FreeStyle™ 293-F Cells (frozen)

• 1 mL 293fectin™ Transfection Reagent

• 100 mL Opti-MEM® I for Complex Formation

• 25 µg pCMV-SPORT β-gal

Please review the FreeStyle™ 293 System Manual for storage and handling information. Store FreeStyle™ 293-F Cells in liquid nitrogen. Store 293fectin™ Transfection Reagents at 4°C. Store GIBCO® FreeStyle™ 293 Expression Medium and Opti-MEM® I at 2-8°C in the dark. Store pCMV SPORT β-gal at -20°C. Reagents are guaranteed stable for six months when stored properly.