Phospho-KIF1B (Ser1487) Antibody

• 产品编号：CST-11993S      品牌：CST       原厂货号：11993S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-KIF1B (Ser1487)抗体可以识别Ser1487被磷酸化的KIF1B蛋白。多抗由合成肽段免疫动物产生，该肽段与人KIF1B蛋白Ser1487及其邻近肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Kinesin-样蛋白KIF1B是C-kinesins 的Kinesin3家族一员，在羧基末端区域有一个Kinesin-motor结构域。作为Kinesin接到的细胞转运机制之一，C-kinesins能够推动微管plus和minus端的活力（1-3）。KIF1B与Rab鸟苷酸交换因子结合促进突触蛋白转运到神经细胞轴突的外围（3）。KIF1B还能转运轴突中的线粒体（3-4）。对有丝分裂磷酸化的定量质谱分析发现了KIF1B的多个潜在的磷酸化位点（5）。Cell Signaling Technology® (CST)使用PhosphoScan®, CST的修饰位点发现平台LC-MS/MS，设计了针对某个位点的Phospho-KIF1B (Ser1487)抗体。使用Akt 第五抗体发现了Ser1487的磷酸化。请访问PhosphoSitePlus®, CST's修饰位点网页，www.phosphosite.org，以获得更多信息。

1. Hirokawa, N. et al. (1998) Curr Opin Cell Biol 10, 60-73.
2. Dagenbach, E.M. and Endow, S.A. (2004) J Cell Sci 117, 3-7.
3. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
4. Wozniak, M.J. et al. (2005) BMC Cell Biol 6, 35.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-KIF1B (Ser1487) Antibody recognizes endogenous levels of KIF1B protein only when phosphorylated at Ser1487.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1487 of human KIF1B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Kinesin-like protein KIF1B is a member of the kinesin 3 family of C-kinesins that are characterized by a kinesin-motor domain in the carboxy-terminal region. As part of the general mechanism of kinesin-mediated cellular transport, C-kinesins are known to drive microtubule plus and minus end motilities (1-3). KIF1B is implicated in the transport of synaptic proteins to the cell periphery in neuronal cell axons by interaction with Rab3 guanine nucleotide exchange factor (3). Mitochondria are also often transported in axons by KIF1B (3-4).Quantitative mass spectrometry profiling of mitotic phosphorylation revealed putative phosphorylation sites of KIF1B (5). Phospho-KIF1B (Ser1487) Antibody is directed at a site identified by Cell Signaling Technology® (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1487 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Hirokawa, N. et al. (1998) Curr Opin Cell Biol 10, 60-73.
2. Dagenbach, E.M. and Endow, S.A. (2004) J Cell Sci 117, 3-7.
3. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
4. Wozniak, M.J. et al. (2005) BMC Cell Biol 6, 35.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.