Human Platelet-Derived Growth Factor BB (hPDGF-BB)

• 产品编号：CST-8912LF      品牌：CST       原厂货号：8912LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > PDGF血小板衍生因子
• 应用分类：

描述：

Cell Signaling Technology的重组人源PDGF-BB(hPDGF-BB)丝氨酸67-苏氨酸190（编号NP_002599）经在E.coli中表达产生。重组的hPDGF-BB再N-氨基末端不含甲基化，经计算分子量为12,294。DTT-还原的蛋白游离体为14kDa多肽，胱氨酸连接的非还原二聚体游离物为32kDa蛋白。重组的hPDGF-BB的预期的N-氨基末端SLGSL由氨基酸测序验证。6 μg还原和非还原的重组hPDGF-BB 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。重组的hPDGF-BB的生物活性由NIH/3T3细胞增殖实验测定。其每个批次的ED50值在 4.0-15 ng/ml之间。每1 μg hPDGF-BB产品所含内毒素少于0.01ng。有载体的：每1 μg hPDGF-BB溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干。无载体的：经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干。PDGF-BB是由胱氨酸连接的二聚体。PDGF家族成员成员在发育、细胞增殖、细胞存活和血管生成中起着关键作用（1,2）。PDGF-BB在血管内皮、巨核细胞和睾丸间质细胞中表达[2]。PDGF-BB靶标外皮细胞、成纤维细胞、内皮细胞和其他类型细胞[1-3]。PDGF 诱导成纤维细胞的生长和迁移[3]。对于单核细胞和粒细胞来说，PDGF是一种化学引诱物。前体的PDGF-BB在胞内被剪切而产生一个含有伸展的C末端的形式，以帮助保持PDGF-BB在细胞外基质。在第二次剪接中，伸展的C末端在胞外被除去而形成PDGF-BB成熟体[1,2]。PDGF-BB 与 PDGFRβ结合诱导受体的二聚化作用并通过PI3K/Akt, JNK,和 PLCγ通路传递信号[1,2]。PDGF-BB可能在某些癌症中起作用[2]。

原厂资料：

Source / Purification

Recombinant human PDGF-BB (hPDGF-BB) Ser67-Thr190 (Accession #NP_002599) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hPDGF-BB does not have a Met on the amino terminus and has a calculated MW of 12,294. DTT-reduced protein migrates as a 14 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 32 kDa protein. The expected amino-terminal SLGSL of recombinant hPDGF-BB was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-BB. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hPDGF-BB was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 4.0 - 15 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hPDGF-BB.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-BB. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1, 2). PDGF-BB may have a role in some cancer types (2).PDGF-BB may have a role in some cancer types (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.