Human Transforming Growth Factor β2 (hTGF-β2)

• 产品编号：CST-8406LF      品牌：CST       原厂货号：8406LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TGF-beta 转化生长因子beta超家族
• 应用分类：

描述：

重组人源TGF-β2 (hTGF-β2) Ala303-Ser414蛋白（Accession #NP_003229） 由Cell Signaling Technology公司人293细胞表达。人源重组TGF- β2不含标签蛋白这种非糖基化蛋白分子量为12719，DTT还原蛋白为12kDa多肽，未还原的由胱氨酸连接而成的二聚体分子量为24kDa。人源重组TGF- β2的预期N-氨基末端的ALDTN由氨基酸测序验证。6 μg还原和未还原的人源重组TGF-β2由 SDS-PAGE测定纯度大于98%，所有批次的产品纯度均大于98%。重组人源TGF-β2的生物活性由抑制IL-4诱导的HT-2细胞的增殖实验来测定。其每个批次的ED50在 0.10-0.3 ng/ml之间。每1 μg hTGF-β1中内毒素含量低于0.01ng每1 μg hTGF-β1溶于含有35%乙腈、0.1%三氟乙酸、20 μgBSA的经0.22 μm滤器过滤的溶液后冻干带有载体。经0.22 μm滤器过滤35%乙腈、0.1%三氟乙酸的溶液的冻干物无载体。TGF-β2由嗜酸性粒细胞、星状细胞、胶质母细胞瘤还有其他癌症来源的细胞产生[1-6]。TGF-β2抑制抗原诱导的T细胞增殖，抑制上皮细胞增殖。诱导间充质细胞增殖并对细胞凋亡有特殊效应[1-8]。TGF-β2与TβRII结合，这种结合由TβRIII促成。TβRI然后与TβRII 和 TβRIII形成复合物[3,5]。其信号转导涉及Smad蛋白磷酸化[2,3,9]，TGF-β2也激活Erk2, p38和JNK 通路[9]。TGF-β2敲除的小鼠心、肺、眼的发育受到严重影响。

原厂资料：

Source / Purification

Recombinant human TGF-β2 (hTGF-β2) Ala303-Ser414 (Accession #NP_003229) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hTGF-β2 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,719. DTT-reduced protein migrates as a 12 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 24 kDa protein. The expected amino-terminal ALDAA of recombinant hTGF-β2 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β2. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hTGF-β2 was was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. 

Endotoxin

Less than 0.01 ng endotoxin/1 μg hTGF-β2

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 35% acetonitrile and 0.1% trifluoroacetic acid containing 20 μg BSA per 1 μg hTGF-β2. Carrier free: Lyophilized from a 0.22 μm filtered solution of 35% acetonitrile and 0.1% trifluoroacetic acid.

Background

TGF-β2 is produced by eosinophils, astrocytes, glioblastoma and other cancer derived cell types (1-6). TGF-β2 inhibits antigen-induced T-cell proliferation, inhibits epithelial cell proliferation, induces mesenchymal cell proliferation and has cell specific effects on apoptosis (1-8). TGF-β2 binds to TβRII and binding is promoted by TβRIII. TβRI then complexes with TβRII and TβRIII (3,5). Signaling involves phosphorylation of the SMAD proteins (2,3,9). TGF-β2 also activates Erk2, p38, and JNK pathways (9). Knockout of TGF-β2 in mice severely impacts heart, lung and eye development (10).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.