Lambda DNA (N6-methyladenine-free)

• 产品编号：N3013L      品牌：New England Biolabs       原厂货号：N3013L
• 产品分类：DNA和RNA纯化 > DNA纯化 > DNAs
• 应用分类：

描述：

Description: 
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (N6-methyladenine-free) is 48,502 base pairs in length. 

Source:
The phage is isolated from a heat-inducible E. coli (dam-) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

Concentration:
500 μg/ml

Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
 

原厂资料：