Mouse Granulocyte Macrophage Colony Stimulating Factor (mGM-CSF)

• 产品编号：CST-5191LC      品牌：CST       原厂货号：5191LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > CSF集落刺激因子
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白GM-CSF (mGM-CSF) Ala18-Lys141 (Accession #NP_034099)是从人源293细胞表达而来。重组的mGM-CSF蛋白在N-末端没有标签，蛋白分子量据推算为14,112.1Da。DTT-还原蛋白作为一个22kDa的蛋白转移。未还原蛋白由于有分子内的胱氨酸存在而作为19 kDa 迁移。在SDS-PAGE中稍低的迁移率和异质性是由于糖基化。重组mGM-CSF 蛋白的N-末端APTRS的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mGM-CSF通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。mGM-CSF的生物活性是通过 MC/9细胞增殖来确定的。每个批次的ED50在6-25pg/ml之间。内毒素含量：小于0.01 ng /1 μg mGM-CSF。

有载体: 每微克mGM-CSF蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mGM-CSF蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

GM-CSF 是通过活化的T细胞，NK细胞和巨噬细胞产生(1,2)。靶标细胞包括粒性白细胞，单核细胞前体和经过分化的一系列的骨髓细胞(1,3,4)。很多的靶标细胞需要GM-CSF才能生存。GM-CSF 诱导增殖，并参与造血细胞的分化成树状细胞，它在干细胞分化的信号通路上也是主要的因素之一。GM-CSF 激活骨髓细胞的功能，所以它连接天然免疫和获得性免疫并推进抗肿瘤免疫(5)。GM-CSF受体由GM-CSFRα和共有的 β链-βC 组成，这同时也被IL-3和 IL-5所利用(1)。GM-CSF的结合起始了 Jak2, Stat5 和 PI3K/Akt 信号通路(1)。

原厂资料：

Source / Purification

Recombinant mouse GM-CSF (mGM-CSF) Ala18-Lys141 (Accession #NP_034099) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mGM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 14,112.1. DTT-reduced protein migrates as a 22 kDa polypeptide and non-reduced protein migrates as a 19 kDa polypeptide due to intramolecular cystines. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal APTRS of recombinant mGM-CSF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mGM-CSF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mGM-CSF was determined in a MC/9 cell proliferation assay. The ED50 of each lot is between 6-25 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg mGM-CSF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mGM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

GM-CSF is produced by activated T cells, NK cells and macrophages (1,2). Target cells include granulocytes, monocyte precursors and subsets of differentiated myeloid cells (1,3,4). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in the hematopoietic differentiation of dendritic cells and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (5). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5 and PI3K/Akt pathways (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.