Human FGF acidic, also known as FGF 1 (fibroblast growth factor 1), is a member of the heparin-binding growth factor family, widely distributed in the peripheral and central nervous systems.
FGF 1 acts as a potent neurotrophic factor, playing a key role during prenatal development and postnatal growth/regeneration and is dependent upon interactions with heparin sulfate (HS) proteoglycans located on cell surfaces and in the extracellular matrix, and the four high affinity tyrosine kinase receptors, designated FGFR-1,-2,-3 and -4.
Bioactivity was determined by the dose-dependent stimulation of thymidine uptake by FGF receptor-expressing BaF3 cells. The ED50 is <10ng/ml.
Target Species
Human
Product Form
Purified - lyophilised
Preparation
Recombinant protein expressed in E. coli.
Preservative Stabilisers
None present
Protein Molecular Weight
15.8 kD (140 Amino Acid Sequence)
Reconstitution
Reconstitute with 50ul of 5mM Sodium Phosphate, pH 8.0
Purity
>95% by SDS PAGE and HPLC analysis
Activity
1 x 105 units/mg
Approx. Protein Concentrations
1 mg/ml after reconstitution
Note
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.