pKLAC1-GLuc Control Plasmid

产品编号：N3034S 品牌：New England Biolabs 原厂货号：N3034S
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 酵母表达载体
应用分类：

包装：

20 μg

运保温度:

-20°C

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描述：

Description:

pKLAC1-GLuc is a yeast reporter plasmid that can be used with the K. lactis Expression Kit (NEB #E1000). The backbone integrative expression vector, pKLAC1, directs high-level secretion of Gaussia priniceps luciferase (GLuc) from the yeast Kluyveromyces lactis. The gene encoding GLuc is cloned in the NotI-StuI sites of pKLAC1, in-frame with the a-factor secretion leader domain. pKLAC1-GLuc can be used to test plasmid transformation, cell culture, and protein production procedures.

Features

Expression of the fusion gene is under the control of the strong K. lactis P_LAC4-PBI promoter.
K. lactis strain GG799 can be used as an expression host.
Gaussia luciferase generates over 1000-fold higher bioluminescent signal intensity, compared toFirefly and Renilla luciferases.
The secreted luciferase protein is very stable and can be stored for several days at 4°C without loss of activity.