BioLux Cypridina Luciferase Assay Kit

• 产品编号：E3309S      品牌：New England Biolabs       原厂货号：E3309S
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 荧光素酶检测
• 应用分类：

描述：

特性;

■   启动子/增强子分析
■  高通量分析
■  转染条件优化
■  与其它报告系统一起进行多重分析
■  分泌途径分析
■  siRNA 筛选
■  时间曲线研究
■  单细胞分析，包括干细胞和原代细胞
■  活细胞分析

概述：NEB 为研究者提供可以检测细胞培养物上清或裂解物中 Gaussia 荧光素酶 (GLuc) 或 Cypridina 荧光素酶 (CLuc) 活性的试剂盒。因为 GLuc 和 CLuc 使用不同的底物，并且无交叉反应，因此，当一种荧光素酶存在时，并不影响另一种荧光素酶的活性测定，这使得这些荧光素酶可作为双重报告系统使用。

BioLux® Gaussia 荧光素酶检测试剂盒含有检测 GLuc 活性所必需的试剂。该试剂盒最常用于直接检测细胞上清液，也可用于检测细胞裂解液。通过精心设计，该试剂盒具有最大的光信号输出。BioLux Gaussia 荧光素酶检测试剂盒也可用来检测海肾荧光素酶的活性，因为 GLuc 和海肾荧光素酶都可以氧化腔肠(coelenterazine) 。

BioLux Gaussia 荧光素酶 Flex 检测试剂盒可以灵活选择较强的荧光信号或较长的信号半衰期。该试剂盒包括较高浓度的底物和一种稳定剂，加入后可以增加信号半衰期。该试剂盒是高通量筛选的理想工具 (见图) 。

BioLux Cypridina 荧光素酶检测试剂盒含有检测 CLuc 活性所必需的试剂。该系统可用于检测细胞培养物上清或细胞裂解物。该系统使用 vargulin 荧光素作为底物。

BioLux Cypridina 荧光素酶启动试剂盒含有两个 CLuc 编码质粒，以及检测 CLuc 活性所需试剂。

原厂资料：

注意事项：

General notes:

1. BioLux Cypridina Luciferase Assay Buffer (1X) and Substrate (lyophilized powder) must always be protected from light.
2. BioLux Cypridina Luciferase Substrate is supplied in lyophilized form to ensure long shelf life and maximum stability.

Usage notes:

1. Store the BioLux Cypridina Luciferase Assay Buffer (1X) at -20°C for up to 1 year.
2. The BioLux Cypridina Luciferase Assay Buffer (1X), the reconstituted CLuc substrate (100X solution) and the CLuc assay solution must be protected from light.
3. The CLuc assay solution should be freshly prepared and incubated at room temperature for 30 minutes (protect from light) before use.
4. The linear range of the luminometer must be established. This can be easily done by assaying serial dilutions of a CLuc containing sample (Figure 3), such as the culture medium of CLuc-expressing cells. In addition, the assay solution itself as well as the conditioned medium (culture medium from untransfected cells) should be included to establish the background in the assay.
5. If excess activity for the instrument range is found, the sample should be diluted in either PBS or 10% serum-containing medium. The integration time can also be reduced, e.g. 2 seconds instead of 5 seconds.
6. The presence of serum, i.e. culture medium, typically increases the background signal in the assay. For example, the CLuc assay solution alone shows 10¹–10² RLU; the CLuc assay solution in 10% FBS-containing medium results in 10²–10⁴ RLU, depending on the type of medium used.
7. The recommended integration time for the luminescence measurement is 2–10 seconds. It is important to keep the integration time constant, in order to obtain consistent results.