EpiMark Hot Start Taq DNA Polymerase

产品编号：M0490L 品牌：New England Biolabs 原厂货号：M0490L
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 热启动PCR
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
EpiMark® Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA.
Taq DNA polymerase possesses a 5´- 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Applications:

PCR from bisulfite-converted DNA

Reagents Supplied:
EpiMark® Hot Start Taq Reaction Buffer Pack (5X)
Heat Inactivation:
No

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol dNTP into acid insoluble material in 30 minutes at 75°C.
Unit Assay Conditions:
1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X EpiMark Hot Start Taq Reaction Buffer, bisulfite-converted DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of EpiMark Hot Start Taq DNA Polymerase in a total reaction volume of 50 µl.
1X EpiMark™ Hot Start Taq Reaction Buffer:
20 mM Tris-HCl
1.8 mM MgCl₂
22 mM NH₄Cl
22 mM KCl
0.06 % IGEPAL® CA-630
0.05 % Tween-20
pH 8.9 @ 25°C
Concentration:
5,000 units/ml
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C