OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer

产品编号：M0489S 品牌：New England Biolabs 原厂货号：M0489S
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 针对高GC含量模板的PCR
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep Vent_R™ DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO₄, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.
Applications:

GC-rich PCR
High Sensitivity PCR
High Throughput PCR
Colony PCR
Long PCR (up to ~6 kb genomic)
Hot Start PCR

Reagents Supplied:
OneTaq High GC Enhancer
OneTaq Hot Start Quick-Load Master Mix with GC Buff

Heat Inactivation:
No

Reaction Conditions:
1X OneTaq Hot Start Quick-Load Master Mix with GC Buffer
1X OneTaq Hot Start Quick-Load Master Mix with GC Buffer:
80 mM Tris-SO₄
Xylene Cyanol FF
20 mM (NH₄)₂SO₄
0.2 mM dNTPs
2 mM MgSO₄
5 % Glycerol
0.05 % Tween-20
5 % DMSO
0.06 % IGEPAL® CA-630
Tartrazine
25 units/ml OneTaq Hot Start DNA Polymerase
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Unit Assay Conditions:
1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X OneTaq Hot Start Quick-Load Master Mix with GC Buffer, DNA template and primers in a total reaction volume of 50 µl.
OneTaq High GC Enhancer:
10 mM Tris-HCl
25% DMSO
25% Glycerol
pH 9.2 @ 25°C
Concentration:
2 X