OneTaq 2X Master Mix with Standard Buffer

• 产品编号：M0482L      品牌：New England Biolabs       原厂货号：M0482L
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 针对高GC含量模板的PCR
• 应用分类：

描述：

Description: 
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent_R™ DNA Polymerases ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl₂ and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.

Applications:

• High Sensitivity PCR
• High Throughput PCR
• Routine PCR
• AT-rich PCR
• Colony PCR
• Long PCR (up to ~6 kb genomic)

Reagents Supplied: 
OneTaq Master Mix with Standard Buffer

Heat Inactivation:
No

Reaction Conditions: 
1X OneTaq Master Mix with Standard Buffer

1X OneTaq Master Mix with Standard Buffer:
20 mM Tris-HCl
22 mM KCl
0.2 mM dNTPs
1.8 mM MgCl₂
5 % Glycerol
0.05 % Tween-20
25 units/ml OneTaq DNA Polymerase
22 mM NH₄Cl
0.06 % IGEPAL® CA-630
pH 8.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions:
1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions:
1X OneTaq Master Mix with Standard Buffer, DNA template and primers in a total reaction volume of 50 µl.

Concentration:
2 X

原厂资料：